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. 2019 Apr 12;9:5965. doi: 10.1038/s41598-019-42435-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorimetric binding assay of wild type LssSH3b_GFP and its variants (N405A, Y472A, N405/Y472) with various strains of S. aureus which represent wild type (pentaglycine) or altered cross-bridges. Results were normalized to the % of binding of wild type LssSH3b_GFP domain to wild type S. aureus NCTC 8325-4 (100%). The error bars represent standard deviation calculated for three experiments each run with triplicates.