Fig. 2.

SRC-1 mediates leptin signaling. Numbers of mice/experiments/neurons are indicated; data are presented as mean ± SEM and compared using T-tests or one- or two-way ANOVA followed by post hoc Sidak tests (#). a Serum leptin levels 42 days after HFD feeding (n = 5/8); *P < 0.05. b Time course of hypothalamic SRC-1-pSTAT3 interaction in C57Bl6 wild type mice that received i.p. injections of leptin (5 mg/kg). c Quantification of the hypothalamic SRC-1-pSTAT3 interaction. *P < 0.05 (#). d Two-hour fasted mice (12 weeks of age) received i.p. injections of saline or leptin (5 mg/kg) 15 min prior to refeeding and food intake was recorded for 1 h afterwards (n = 7/9); **P < 0.01 (#). e Representative pSTAT3 immunohistochemical staining in the ARH and VMH of control and pomcSRC-1-KO mice receiving a single bolus i.p. injection of leptin (0.5 mg/kg, 90 min). Scale bar = 50 μm. 3V the 3rd ventricle, ARH arcuate nucleus, VMH ventromedial hypothalamic nucleus. f Quantification of pSTAT3 (+) neurons in the ARH (n = 5); ***P < 0.001. g Representative traces of leptin-induced depolarization, in the presence of TTX, CNQX, DAP-5, and bicuculline, in mature Pomc neurons from control mice vs. from MpomcSRC-1-KO mice after 1-week HFD feeding. h Responsive ratio (depolarization is defined as >2 mV elevations in resting membrane potential) (n = 39/43); P = 0.002 in χ² tests. i Quantification of leptin-induced depolarization in two groups (n = 39/43); **P < 0.01. j Representative traces of action potentials in untreated mature Pomc neurons from control mice vs. from MpomcSRC-1-KO mice. k, l Quantification of firing frequency (k) and resting membrane potential (l) in two groups (n = 29–36); *P < 0.05. m Representative traces of mIPSC in untreated mature Pomc neurons from control mice vs. from MpomcSRC-1-KO mice. n, o Quantification of amplitude (n) and frequency (o) of mIPSC in two groups (n = 13/14); ***P < 0.001. Source data are provided as Source Data Fig. 2