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. 2019 Apr 12;9:5984. doi: 10.1038/s41598-019-42401-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of T_RM signature genes. (A) Typical example of the gating strategy used after fluorescence activated CD8+ cell sorting to obtain recirculating T cells (CD69−CD103−), CD103− T_RM cells (CD69+CD103−), and CD103+ T_RM cells (CD69+CD103+). Cells were gated by forward- and side-scatter followed by 7-AAD negative (viable) gating. (B) Heatmaps depicting the normalized gene expression of eight signature genes known to be upregulated (yellow) or downregulated (blue) in T_RM cells of the renal allograft and spleen. Spleen n = 5, renal lymphocytes n = 4.