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. 2019 Apr 12;8(4):28. doi: 10.1038/s41389-019-0138-y

Fig. 3. Inhibitory effect of ZFHX3 on colony formation depends on the downregulation of MYC in prostate cancer cells.

Fig. 3

a Expression of MYC in prostate cancer cell lines, as detected by Western blotting. b Knockout or knockdown of ZFHX3 upregulated MYC expression in C4-2B and LNCaP cells, as detected by Western blotting. Wt, KO3, and KO8 are vector control and two ZFHX3-null clones of C4-2B. c–e Knockdown of MYC, by using siRNAs against MYC at two concentrations (20 and 40 nM), eliminated the promoting effect of ZFHX3’s loss on cell proliferation and colony formation in soft agar in C4-2B cells. The SRB assay was used to measure cell proliferation (d), while the soft agar assay was used for colony formation (e). The n of both d and e is 4. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. ZFHX3, zinc-finger homeobox 3; siRNA, small interfering RNA; Wt, wild type; SRB, sulforhodamine B