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. 2019 Apr 12;9:5995. doi: 10.1038/s41598-019-41921-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Body weight and composition of GPRC6A knockout (KO) mice and wildtype (WT) littermates. Total fat (A) and lean body mass (B) were measured by MRI in vehicle (veh)- and corticosterone (CS)-treated animals on day 0 and 28. There was no significant difference between the two vehicle-treated groups or between CS-treated WT and KO animals. (C) Changes in compartmental fat at endpoint demonstrated no significant differences between genotypes. (D) Body weight measured at the indicated ages. Data are presented as mean ± S.E.M. (n = 6–8). Data were analyzed by two-way ANOVA with Tukey’s post hoc test (A–C) or Student’s t-test (D). *P < 0.05, **P < 0.01, ***P < 0.001.