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. 2019 Apr 12;10:1726. doi: 10.1038/s41467-019-09665-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — TET3 is highly expressed in adult NSCs in vivo and in vitro. a qPCR for Tet1, Tet2 and Tet3 genes in different cell types and tissues. Tet1 is low expressed in adult NSCs and Tet2 downregulates postnatally. Tet3 is highly expressed in the NSC population and maintained postnatally. Significant levels of Tet3 were also observed in NSCs after 2, 3 and 7 days (DIV) of differentiation in vitro. P: postnatal day. Embryonic stem cell lines (ESCs) were used as a control of expression. Data are expressed relative to Gapdh. Mean is indicated in the boxplots as “+”. b Immunohistochemistry images for TET3 (red), GFAP (green), and SOX2 (blue) in the SVZ of adult wild-type mice. High-magnification images are shown. Dark arrowheads indicate triple positive astrocytes cells. V lateral ventricle lumen. c Immunocytochemistry for TET3 (red) and SOX2 (green) in proliferating neurospheres isolated from the adult SVZ. d Immunocytochemistry for TET3 (red) and Nestin (green) or GFAP (green) in adult NSCs after 3 and 7 days of differentiation. DAPI was used to counterstain DNA. One-way ANOVA and Tukey post-test were applied. All error bars show s.e.m. Number of samples used is indicated. Scale bars, 20 µm (high-magnification images, 7 µm). Source data are provided as a Source Data file