Fig. 5.

BMP2 mediates terminal differentiation of Tet3-deficient NSCs. a qPCR for Bmp2 in Tet3-Gfap^control and Tet3-Gfap^cre interfered NSCs after 2 DIV of differentiation. Data are expressed relative to Gapdh. b Percentage of pSMAD1/5+cells relative to total cells in Tet3-Gfap^control and Tet3-Gfap^cre NSCs that have been interfered with a shRNA for SNRPN (left). NSCs were treated with 100 ng/ml of the BMP natural antagonist Noggin. Immunocytochemistry images for pSMAD1/5+cells (right). DAPI was used to counterstain DNA. c BRE-tk-luciferase reporter activity in Tet3-Gfap^control and Tet3-Gfap^cre shRNA interfered neurospheres and treated with Noggin. All error bars show s.e.m. One-way ANOVA and Tukey post-test were applied. P-values and number of samples are indicated. Mean is indicated in the box and whiskers plots as “+”. Scale bars in (b), 40 µm (high-magnification images 15 µm). Source data are provided as a Source Data file