Fig. 1.

Steady state de novo proteome in the hippocampus of wild-type and Fmr1 knockout mice. a Schematic timeline and workflow of the BONLAC experiment in hippocampal slices from wild-type (WT) and Fmr1 knockout (KO) mice. Data are from 3 biological replicate experiments (n = 3 mice/treatment/experiment for 3 separate experiments, therefore n = 9/genotype split pooled into groups of 3). b Fold change vs intensity distribution plot of all detected proteins in KO versus WT hippocampal proteome in black. Overlay with red scatter plot depicts candidate proteins that were consistently altered using cut-offs derived from C-score rank score algorithm. c BinGO Cytoscape depiction of enriched gene ontology groups. d Top functional clusters in Fmr1 KO mice compared to WT littermates using DAVID 6.8. e AHA-pull down validations (n = 3 mice per genotype in WT/KO sets)