Table 3:
Sequence motifs that compose the 25 different target primers for the first and the second PCR. First PCR primers consist of target-specific primer followed by an overhang out of sample-specific Tag 1 and Read 1 and Read 2 sequencing primer, respectively. The second PCR primers consist of the Read 1 or the Read 2 sequencing primer followed by a plate-specific Tag 2 and the P5 and P7 adapters, respectively (see also Fig. 2).
| Name | Sequence | Reference |
|---|---|---|
| Tag A | TGCAT | [50] |
| Tag B | TCAGC | [50] |
| Tag C | AAGCG | [50] |
| Tag D | ACAAG | [50] |
| Tag E | AGTGG | [50] |
| Tag F | TTGAC | [50] |
| Tag G | CCTAT | [50] |
| Tag H | GGATG | [50] |
| Tag I | CTAGG | [50] |
| Tag K | CACCT | [50] |
| Tag L | GTCAA | [50] |
| Tag M | GAAGT | [50] |
| Tag N | CGGTT | [50] |
| Tag O | ACCGA | [50] |
| Tag P | ACGTC | [50] |
| Tag Q | AGACT | [50] |
| Tag R | AGGAA | [50] |
| Tag S | ATTCC | [50] |
| Tag T | CAATC | [50] |
| Tag V | CATGA | [50] |
| Tag W | CCACA | [50] |
| Tag X | GCTTA | [50] |
| Tag Y | GGTAC | [50] |
| Tag Z | AACAC | [50] |
| Tag Control | ATCTG | [50] |
| CytB-fw | AAAAAGCTTCCATCCAACATCTCAGCATGATGAAA | [44] |
| CytB-rv | AAACTGCAGCCCCTCAGAATGATATTTGTCCTCA | [44] |
| 16S-fw | CGGTTGGGGTGACCTCGGA | [45] |
| 16S-rv | GCTGTTATCCCTAGGGTAACT | [45] |
| 12S-fw | AAAAAGCTTCAAACTGGGATTAGATACCCCACTAT | [44] |
| 12S-rv | TGACTGCAGAGGGTGACGGGCGGTGTGT | [44] |
| Read 1 sequence primer | ACACTCTTTCCCTACACGACGCTCTTCCGATCT | Illumina Document 1000000002694 v03 |
| Read 2 sequence primer | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT | Illumina Document 1000000002694 v03 |
| P5 adapter | AATGATACGGCGACCACCGAGATCTACAC | Illumina Document 1000000002694 v03 |
| P7 adapter | CAAGCAGAAGACGGCATACGAGAT | Illumina Document 1000000002694 v03 |