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. 2019 Apr 13;7(7):e14069. doi: 10.14814/phy2.14069

Figure 2.

Figure 2

TRPV4 deficiency prevents PgLPS‐induced exacerbation of foam cell formation in response to increased matrix stiffness. (A) WT and TRPV4−/− MRMs were grown on collagen‐coated (10 μg/mL) soft (0.5 kPa) and stiff (8 kPa) polyacrylamide gels for 24 h with or without PgLPS (500 ng/mL), followed by incubation with oxLDL (50 μg/mL) for 24 h. Oil‐Red‐O staining was performed to examine foam cell generation. Representative images from five different fields per condition are shown (original magnification, 40×). (B) Bar graph shows quantitation of results in Figure 2A. Results are expressed as mean ± SEM. **P < 0.01 for TRPV4−/− cells on 8 kPa versus WT on 8 kPa; ***P < 0.001 for TRPV4−/− on 8 kPa with PgLPS versus WT cells on 8 kPa with PgLPS; ## P < 0.01 for WT cells on 8 kPa versus WT cells on 8 kPa with PgLPS; n = 50 cells/condition, Student's t‐test.