Fig. 4.

NSAIDs exisulind and nicotinamide treatment, but not ketoprofen increases p53 activity in vivo. Five female mice per group were treated per os (gavage) with a daily dose of 3.75, 7.5, 15 mg/Kg exisulind (exi), 15 mg/Kg nimesulide (nim), 15 mg/Kg ketoprofen (ket), 15 mg/Kg nicotinamide (NAM) or dimethyl sulfoxide (DMSO, vehicle). Treatment was carried out for eight days; at day 5 a single dose of an acetone solution of 12 mM DMBA (left mammary gland) or acetone (right mammary gland) was injected in the mammary fat pad of the animals (Figure S4B). p21 mRNA expression was determined by real-time PCR; bars in the graph are the average ± SEM values quantified with the 2^−∆∆Ct method. * P < 0.05; ** P < 0.01 DMBA versus acetone treated breast. P-values were calculated by Student’s t-test