Fig. 6.

Intraoperative ketorolac induces p53 acetylation at the K382 site in clinical settings. a Model of the NSAIDs and exisulind chemopreventive action: the direct inhibition of SIRT1 leads to the unbalance activity of p53 involved in the early steps of tumourigenesis. A genotoxic stress increases proliferation (left panel, − NSAIDs) which is counteracted by NSAIDs (right panel, + NSAIDs). b Immunoblot analysis was carried out using anti-acetyl (K382) p53 and anti-total p53 antibodies and protein extracts obtained from 13 patients: six were treated with ketoroloac (20 mg per os) 2 h before surgery and seven with opiates for intraoperative analgesia. β-actin is reported as loading control. Bars in the graphs represents densitometry quantifications of the autoradiographic signals (acetylated p53 vs total p53); ***P < 0.001 versus the level of control; P-values were calculated by Student’s t-test