Fig. 2.

NAc-iLTD is postsynaptically expressed. a Representative traces of spontaneous IPSC events (sIPSC) before (black) and after NAc-iLTD induction (gray). Scale bars: 200 pA, 50 s. b The cumulative frequency distribution (CFD) of sIPSC amplitudes after NAc-iLTD induction (gray) differed from baseline (black). c The CFD of the frequency of sIPSC events before and after NAc-iLTD induction were not different. d Top: The paired-pulse ratio (PPR) before (black) and 25 min after (gray) NAc-iLTD induction were not different, but was different the first 5 min after induction (red). Open squares are individual cells; filled squares represent the mean PPR. Bottom: Representative traces at each time point. e Top: The coefficient of variation (CV) did not differ before and 25 min after LFS delivery. CV was significantly different the first 5 min following LFS delivery. Bottom: Representative traces at each time point; the red trace is the average of all the traces at that time point. f Top: PPR at minutes 6–10 and 25–30 did not differ following BDNF application. Bottom: Representative traces at each time point. g Top: CV of IPSC amplitude following BDNF application at minutes 25–30 did not differ from baseline values or minutes 6–10. Bottom: Representative traces at each time point; the red trace is the average of all the traces at that time point. h Including the MEK inhibitor, U0126 (2 µM) in the internal pipette solution blocked NAc-iLTD induction (red), compared to controls (black). i Inclusion of the PLC inhibitor, U73122 (1 µM) in the internal pipette solution did not eliminate NAc-iLTD. j Inhibiting protein synthesis with cycloheximide (25 µM) in the artificial cerebrospinal fluid (aCSF) did not alter NAc-iLTD expression. k Inhibiting dynamin-mediated endocytosis with a dynamin-inhibitory peptide (DIP, 50 µM) in the internal pipette solution blocked NAc-iLTD (red), while including a scrambled version of this peptide (DIPS, 50 µM) did not (black). Insets: Representative traces of the first 5 min (dark) and final 5 min (light) of the experiment. Scale bars: 200 pA, 200 ms, unless noted otherwise. *p < 0.05. Data are represented as mean ± SEM