Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 15;120(5):512–521. doi: 10.1038/s41416-018-0373-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — The relationship between epithelial-to-mesenchymal transition (EMT) and Axl activation in the development of acquired sorafenib resistance in immortalised hepatocellular carcinoma (HCC) cell lines. a Sulphorhodamine-B (SRB) assays demonstrating a significant shift in the GI₅₀ following chronic sorafenib incubation in HuH7SR and SKHep-1SR compared to their parental counterparts. b, c Migration assays demonstrating a significant increase in cell motility following the acquisition of sorafenib-resistance in HuH7 and SKHep-1 cells. d Antibody array experiments demostrating the differential activation of 28 RTK and 11 signalling nodes in sorafenib-resistant cell clones compared with naive counterparts. A 4-fold increase in Axl phosphorylation is noted in SKHep-1SR cells compared to their sorafenib-naive counterparts. e Analysis of the expression of Axl as well as key EMT-related proteins and Axl-related signalling nodes demonstrating a significant increase in Axl and Met phosphorylation in SKHep-1SR cells and significant overexpression of E-cadherin, Vimentin, β-Catenin together with Claudin repression in HuH7SR cells suggesting differential activation of EMT across cell lines in the context of sorafenib resistance. Representative replicates of at least 3 experiments are shown. ***p < 0.001