Fig. 3.

UA promoted cardiac development in the early stage of differentiation. a Schematic of cardiac differentiation. C01: CDM3-UA + CHIR99021; C02: CDM3-UA + Wnt-C59; C03: CDM3-UA. D, day. b Schematic diagram of the differentiation protocols. c Efficiency of cardiac differentiation measured by flow cytometry for EGFP⁺ on day 15 cells in different differentiation protocols. The entire group compared with the group of C01C02C03 (-). d NKX2–5-GFP hESC cells were treated with differentiation protocols for 15 days under inverted microscope. EGFP expression was analyzed under fluorescent microscopy with a fixed exposure time. Scale bars = 200 μm. e Percentages of EGFP⁺ cardiomyocytes at day 15 in the differentiation protocols. n = 3 each. Data are expressed as means ± SD. *P < 0.05, **P < 0.01 vs. control