Fig. 1.

Compound-1 had lower agonistic effect than Compound-2 in primary neurons. a Chemical structures of Compound-1 and Compound-2. b Displacement studies with Compound-1 and Compound-2 by SPA using [³H]-HBT1 and His-LBD. c Displacement studies with Compound-1 and Compound-2 by binding assay using [³H]-HBT1 and hippocampal membranes. Data (b, c) are presented as the mean ± SD (n = 3–4). d Effects of Compound-1 and Compound-2 on Ca²⁺ influx in GluA1i CHO cells in the presence or absence of 3 mM glutamate. e Effects of Compound-1 and Compound-2 on Ca²⁺ influx in primary neurons in the presence or absence of 5 μM AMPA. The experiments were repeated at least twice, and representative graphs (d, e) are shown. Data are presented as mean ± SD (n = 3). f Effects of Compound-1 and Compound-2 on [³H]-AMPA binding to rat hippocampal membranes. Data are presented as mean ± SD (n = 3)