Fig. 2.

NDR depletion abolishes EMT induced by RASSF1A silencing in HBEC cells. HBEC-3 (a) or A549 (b) cells were transfected with siNeg, siRASSF1A and/or with siNDR1 or siNDR2. c-d Xenograft obtained from shcontrol, shNDR1 or NDR2 A549 cells. Quantification of E-Cadherin, syndecan-1, ZO-1, vimentin and/or N-cadherin by western blot (a, c), Immunofluorescence (b) or immunohistochemistry (d). Error bars indicate the SEM (n ≥ 3). *P < 0.05, **P < 0.01 and ***P < 0.001, using an ANOVA test followed by Dunnett’s test