Fig. 3.

NDR depletion abolishes YAP activation induced by RASSF1A silencing in HBEC cells. a-b HBEC-3 cells were transfected with siNeg, siRASSF1A and/or with siNDR1 or siNDR2. c-d Xenograft obtained after subcutaneous injection of shcontrol, shNDR1 or NDR2 H1299 cells. Nuclear YAP quantification by immunofluorescence (a) or by immunohistochemistry (c). b, d Quantification of CTGF (Bi, Di) & ANKRD1 (Bii, Dii) mRNA using actin as an internal control in HBEC-3 cells. Error bars indicate the SEM (n ≥ 3). *P < 0.05, **P < 0.01 and ***P < 0.001, using an ANOVA test followed by Dunnett’s test