Fig. 5.

NDR2 and GEF-H1 are co-stained in HBEC-3 cells during both interphase and cell division. HBEC-3 cells were transfected with siNeg or siRASSF1A. a GEF-H1 and NDR2 co-staining assayed by immunofluorescence in HBEC-3 cells during interphase. b Representative images are shown for NDR2 and GEF-H1 during mitosis. Localization was identified by immunofluorescence and confocal microscopy. Costaining was evaluated by ImageJ software. HBEC-3 cells were also stained with DAPI for DNA and PSer885GEF-H1 during mitosis. Error bars indicate the SEM (n ≥ 3). *P < 0.05, **P < 0.01 and ***P < 0.001, using an ANOVA test followed by Dunnett’s test