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. 2019 Apr 12;38:158. doi: 10.1186/s13046-019-1145-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — GEF-H1 overexpression as NDR depletion restores cytokinesis of RASSF1A depleted human bronchial cells. HBEC-3 (a-c) were transfected with siNeg, siRASSF1A and/or with siNDR1, siNDR2 or pcB6-GEF-H1 (a). The number of binucleate (a, c) and interconnected cells (b, c) were counted after alpha-tubulin and DAPI staining from cells over-expressing or not GEF-H1 (a) or silenced for NDR kinases (c) in HBEC-3. These numbers are expressed as a percentage in control and siRNA-transfected cells. Error bars indicate the SEM (n ≥ 3). *P < 0.05, **P < 0.01 and ***P < 0.001, using an ANOVA test followed by Dunnett’s test