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. 2019 Apr 13;16:85. doi: 10.1186/s12974-019-1468-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of MIF inhibitor 4-IPP on the expression of PGE₂ synthesis-related protein. a Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 100 μM 4-IPP. b–d Quantification data as shown in (a). e, f Cell supernatants (e) and lysates (f) were tested by ELISA for the production of PGE₂, following astrocytes treatment with 0–2.5 μg/ml recombinant MIF for 24 h in the presence of 100 μM 4-IPP. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)