Table 1.
Dilution ratios of primary antibodies against GPR40, IL-10, β-endorphin, Iba-1, NeuN, and GFAP in immunocytochemistry and immunohistochemistry
| Primary antibody | Manufacturer | Dilution ratio | |
|---|---|---|---|
| Cell flask | Tissue section | ||
| GPR40 | Santa Cruz Biotech, Santa Cruz, CA, USA | 1:100 | 1:50 |
| IL-10 | R&D system, Minneapolis, MN, USA | – | 1:200 |
| β-Endorphin | Phoenix Pharmaceuticals, Burlingame, CA, USA | – | 1:100 |
| Iba-1 | Merck Millipore, Darmstadt, Germany | 1:300 | 1:100 |
| NeuN | Merck Millipore, Darmstadt, Germany | 1:100 | 1:60 |
| GFAP | Merck Millipore, Darmstadt, Germany | 1:200 | 1:100 |