Fig. 4.

Cwf19L2 may play an important role during spliceosome disassembly and intron lariat RNA debranching. a In the ILS1 complex, Cwf19L2 interacts closely with the lariat junction at the splicing active site and directly contacts the RNaseH-like (wheat), Linker/Endo (light blue), and N-domain (gray) of Prp8. Shown in the left is a cartoon representation of key components at the active site. Shown in the right is a surface representation of these components in a related view. b A close-up view on the splicing active site. Prp8 is hidden except the 1585-loop (colored blue), which is located beneath the “clamp” of Cwf19L2. c Cwf19L2 employs a positively charged surface to interact with the RNA elements. The electrostatic surface potential is shown for Cwf19L2. Two perpendicular views are presented