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Establishment of DDIT4 gene-suppressed and gene-overexpressing glioblastoma cells. a, b LNT-229 and G55 pSuper Puro DDIT4sh and control (non-targeting sequence, NTsh) cellular lysates were analysed by immunoblot with antibodies for DDIT4 or actin. cDNA was analysed by qPCR. c G55 DDIT4 Tet-off cells were incubated for 3 days in serum-free DMEM with and without 1 μg/ml doxycycline. Cellular lysates were analysed by immunoblot with antibodies for DDIT4 or actin. cDNA was analysed by qPCR
