Fig. 5.

Ezrin interacts with AKT in BC. a The protein expression levels of p-AKT analysed by Western blot after knockdown or overexpression of Ezrin in BC cells. b Ezrin interacts with AKT. Lysates from MDA-MB-231 and MCF-7 cells were subjected to immunoprecipitation and western blot with the indicated antibodies. An irrelevant IgG was used as the IP control. c The correlation of Ezrin and p-AKT in the xenograft tumours tissues was detected by IHC. d AKT was knockdown in Ezrin overexpression cells. e Cell proliferation was examined by colony formation assay in constructed cells with or without AKT knockdown. f Cell invasion were investigated by transwell assays in constructed cells with or without AKT knockdown. g Western blot analysis of EMT markers and VEGF in constructed cells with or without AKT knockdown