Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 6;120(5):527–536. doi: 10.1038/s41416-018-0315-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Inhibition of MET impairs IFNγ-induced PD-L1 expression in MET-amplified organoids derived from metastatic CRC patients. a Immunofluorescence analysis of PD-L1 expression and phospho-MET status in M162 and CRC1169 organoids treated for 48 h with IFNγ (50 ng/ml) alone or in combination with JNJ-605 (50 nM). NT untreated cells. Upper panels: representative confocal sections showing PD-L1 (green), phalloidin (red) and DAPI (blue). Lower panels: representative confocal sections showing phospho-MET (green), total MET (red) and DAPI (blue). b Graphs reporting the ratio of mean pixel fluorescence intensity (MFI) between PD-L1 and phalloidin or the ratio of mean pixel fluorescence intensity between phosphoMET and total MET, background subtracted. Each point is the mean of five values ± SEM. Bar is 50 µm. (**, P ≤ 0.01; *, P ≤ 0.05; ns not statistically significant)