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. 2018 Apr 5;16(4):401–409. doi: 10.1038/s41423-018-0026-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Re-modulation of the tumor microenvironment by low-dose DAC. a The mice were inoculated with the tumor, and beginning 7 days later, each mouse received peritoneal injections of 20 μg DAC for 5 consecutive days. The last day of injection was recorded as DAC-0 day. The mice were sacrificed at DAC-1 day, DAC-3 day, DAC-7 day and DAC-14 day. The tumor sections were stained for CD3 by IHC. b The stained slides were analyzed using the PerkinElmer Vectra 3 automated, high-throughput quantitative pathology imaging system. CD3-positive T cells were counted under ×200 magnification. From each slide, 10 fields were selected for analysis. The results were analyzed using ANOVA. *p < 0.05, ***p < 0.001, ns indicates no significant difference. c The tumors on DAC-7 day from the PBS-treated, Cytidine-treated, and DAC-treated mice were stained for CD3, CD4, CD8, IFN-γ and PD-1. d The stained slides were analyzed using the PerkinElmer Vectra 3 automated, high-throughput quantitative pathology imaging system. The positive cells were counted under ×200 magnification. From each slide, 10 fields were selected for analysis. The results were analyzed using ANOVA. ***p < 0.001