Fig. 5.

MST1 depletion increases nuclear Yes-associated protein (YAP) but decreases nuclear transcriptional co-activator with PDZ-binding motif (TAZ). a–d MST0-211H, H2452, and H2052 cells were transfected with siNeg, siMST1, or siMST1+pcDNAMST1. Experiments performed 48 h after transfection. a YAP nuclear and cytoplasmic localisation assayed by immunofluorescence. YAP/TAZ activity by quantifying ANKDR1 expression in b H2452 or c H2052 cells. d Quantification of TAZ, YAP, and PSer127-YAP protein levels using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal control. For all histograms, error bars indicate the SEM of at least three independent experiments; *p < 0.05, **p < 0.01, and ***p < 0.001, using an analysis of variance (ANOVA) test followed by Dunnett’s test