Fig. 3.

PMP uptake reduces the release of NO from endothelial cells. a Uptake of DiO-labeled PMPs (green) by HUVECs is shown using immunofluorescence. HUVECs were stained with CD31 (red), and the nuclei were labeled with DAPI (blue, scale bar = 50 μm). b Effects of isolated plasma microparticles from diabetic rats on the production of endothelial NO. Ctrl-PMPs plasma PMPs isolated from the Control group, DM-PMPs, plasma PMPs isolated from the DM group; Aspirin-PMPs, plasma PMPs isolated from the DM + Aspirin group. The results represent the mean values ± SEM (n = 8). *P < 0.05 versus Ctrl-PMPs. c Effects of PMPs (10⁴/mL to 10⁷/mL) obtained from activated platelets on the production of endothelial NO. The results represent the mean values ± SEM (n = 8). **P < 0.01 versus Control, ***P < 0.001 versus Control