Fig. 3.
The D motif of Mce2E contributes to innate immune suppression during mycobacterial infection in vivo. a, b Quantitative PCR analysis of Tnf mRNA (a) and Il6 mRNA (b) in splenic cells from mycobacteria-infected mice. C57BL/6 mice were infected intratracheally with 2 × 106 of the WT BCG, BCG Δmce2E, BCG Δmce2E:mce2E, or BCG Δmce2E:mce2E AAA strain for 0–20 days. c, d Hematoxylin and eosin (H&E) staining of the lungs (c) and livers (d) of mice treated as in a and b. Arrows indicate foci of cellular infiltration. Scale bars, 200 μm. e Bacterial load in homogenates from the lungs of mice treated as in a and b. *P < 0.05 and **P < 0.01 (unpaired two-tailed Student’s t test). Data are representative of experiments with two independent biological replicates (mean and s.e.m. of n = 6 mice per group)