Fig. 3. Type-III IFN-induced CBFβ production is IL-10 dependent.

a HepG2 cells were transfected with pHBV1.3 or left untransfected as indicated; RNA was extracted and subjected to Q-PCR analysis to measure IL-10 or IL-27. b The supernatant from A was evaluated to detect the IL-10 level by ELISA. c HepG2 cells were treated with IFN-λ1 as indicated; Q-PCR or ELISA was performed to analyze the IL-10 mRNA or protein level. d HepG2 cells were treated with IFN-λ1 or together with IFN-λ1 or IL-10-neutralizing antibody as indicated, whole-cell lysates were immunoblotted with CBFβ or GAPDH antibodies, and RNA was extracted and subjected to Q-PCR analysis to measure CBFβ expression. e HepG2 cells were transfected with siRNA control or siIL-10 as indicated and analyzed as in d. f HepG2 cells were treated with IL-10 protein as indicated and analyzed as in d. All of the data are from three pooled independent experiments and are shown as the mean ± s.d. *p < 0.05,**p < 0.01, ***p < 0.001, ^#p > 0.05