Fig. 4. CBFβ inhibits HBV replication.

a, c HepG2 cells were pretreated with IFN-λ1 for 8 h as a positive control or left untreated; after treatment, cells were transfected with pHBV1.3 or cotransfected with pHBV 1.3 and with different amounts of CBFβ as indicated. At 72 h, the supernatant was collected, and cells were subjected to immunoblotting with antibodies as indicated. RNA and HBV DNA were isolated from cells or supernatants and analyzed by Q-PCR. HBeAg in the supernatant was analyzed by ELISA. b, d HLCZ-01 cells were transfected with CBFβ or empty vector together with GFP plasmids; 36 h later, GFP-expressed cells were sorted to 24-well plates and infected with HBV. Then, 7 days after infection, the cells and supernatants were collected and analyzed by Q-PCR or ELISA, and the cells were subjected to immunoblotting with antibodies as indicated. All of the data are from three pooled independent experiments and are shown as the mean ± s.d. *p < 0.05, **p < 0.01