Fig. 5. CBFβ is essential for IFN-λ-mediated inhibition of HBV.

a HepG2 WT and CBFβ KO cells were transfected with pHBV1.3 or cotransfected with pHBV 1.3 and CBFβ. Cells and supernatants were collected at 72 h; HBV DNA was isolated from the supernatants and analyzed by Q-PCR. HBeAg in the supernatant was analyzed by ELISA. Data are presented as the means and s.d. from three independent experiments. Student’s t-test was performed. **p < 0.01. b HepG2 WT and CBFβ KO cells were pretreated with IFN-λ1 for 8 h and transfected with pHBV1.3; 48 h later, the cells and supernatants were collected and analyzed as in a. All of the data are from three pooled independent experiments and are shown as the mean ± s.d. *p < 0.05, **p < 0.01