Fig. 4.

Enoxacin rescues ZIKV-caused microcephaly in brain organoids. Following ZIKV infection, D20 brain organoids treated with enoxacin were subjected to immunostaining and virological analysis. a Enoxacin treatment prevented ZIKV infection and the thinning of the VZ layer in the D20 organoids as indicated by the white dotted lines. ZIKV E (green), SOX2 (red), TUJ1 (blue). ZIKVE⁺ cells and the thickness of the SOX2⁺ VZ layer were quantified. Scale bar, 50 μm. b Morphologic demonstration of the organoids in each group. Surface areas of organoids were quantified. All data are means ± SD (n = 5 organoids) of three independent experiments, Student’s t-test and one-way ANOVA, ***P < 0.001, ****P < 0.0001. Scale bar, 200 μm