Fig. 1.

Three cycles of Binge-Like Ethanol Intake Reduces mPFC NPY Expression. a Timeline of three cycles of DID ethanol or water exposure. b Three cycles of binge-like ethanol intake reduced NPY immunoreactivity relative to animals exposed only to water in the prelimbic (PL) and infralimbic (IL) subregions of the mPFC. c Representative images of NPY IHC in the PL and IL in each treatment group. d NPY immunoreactivity and total binge-like ethanol consumption on the final day of cycle 3 (g/kg) were found to be significantly correlated in the PL, e but not the IL. f NPY immunoreactivity and total water consumption on the final day of cycle 3 (ml/kg) did not correlate in the PL g or IL subregion of the mPFC. (* = main factor: liquid p < 0.05) (scale bar = 100 µm)