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. 2019 Mar 11;29(4):313–329. doi: 10.1038/s41422-019-0153-8

Fig. 1.

Fig. 1

AdoCbl inhibits LRRK2 kinase activity. a Domain structure of LRRK2. b Dose-response curves of brain-purified flag-tagged LRRK2 kinase as a function of different forms of cobalamin. Phosphorylation is quantified by measuring TR-FRET emission ratios of fluorescein-LRRKtide and a Terbidium-labeled pLRRKtide antibody. c Dose-response curves of strep-tagged LRRK2 autophosphorylation or d phosphorylation of myelin basic protein as a function of different forms of cobalamin. e Dose-response curve of strep-tagged LRRK2-G2019S phosphorylation of purified Rab10 as a function of AdoCbl. f Dose-response curves of pS935/Total LRRK2 and g pS1292/Total LRRK2 after treatment with different forms of cobalamin in MEF cells derived from LRRK2-G2019S BAC transgenic mice. Data from each replicate were normalized to LRRK2 phosphorylation without cobalamin treatment. All data points represent the mean (±s.d.) of three biological replicates