Fig. 1.

PKM2 is positively related to HBXIP in clinical breast cancer patients. a IHC staining of HBXIP and PKM2 in the normal breast tissues (N) and breast carcinomas (T1, T2) from breast tissue microarray. Scale bar, 100 μm. The association between HBXIP and PKM2 expression was statistically significant, analyzed by Pearson χ² independence test, χ² = 32.34, P < 0.001. b Heatmap of the expression of HBXIP and PKM2 in above breast tissue microarray. Numbers 0, 1, or 2 represent the negative, moderate, or intense staining, respectively. c Relative mRNA levels of HBXIP and PKM2 in 34 clinical breast tumor tissues tested by real-time PCR assay. The correlation between HBXIP and PKM2 at the mRNA levels was determined by Pearson’s correlation coefficient. Statistically significant differences are indicated: ***P < 0.001; all experiments were repeated at least three times