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. 2018 Jun 20;40(4):530–538. doi: 10.1038/s41401-018-0015-9

Fig. 2.

Fig. 2

The expression of PKM2 can be upregulated by HBXIP in ER+ breast cancer cells. a, b Real-time PCR assay of PKM2 mRNA level in MCF-7 (a) and MCF-7-HBXIP (b) cells transiently transfected with indicated plasmids or siRNA for 48 h. c, d RT-PCR assay of PKM2 mRNA level in MCF-7 (c) and MCF-7-HBXIP (d) cells transiently transfected with indicated plasmids or siRNA for 48 h. e, f Immunoblotting assay of PKM2 protein level in MCF-7 (e) and MCF-7-HBXIP (f) cells transiently transfected with indicated plasmids or siRNA for 48 h. Statistically significant differences are indicated: *P < 0.05, **P < 0.01; all experiments were repeated at least three times