Fig. 1.

Dynamic changes in miR-135a-5p expression in the lung tissue of the PAH rat model and in vitro hypoxic exposure. a, b The levels of miRNAs, including miR-135a-5p, miR-211-5p, miR-204-5p, miR-224-5p, miR-93-5p, miR-17-5p, and miR-27b-5p in rat lung tissue at the 4th week after MCT treatment or hypoxic exposure via real-time PCR. c, d The expression level of miR-135a-5p in the lung tissue of rats at the indicated time points after MCT treatment or hypoxic exposure via real-time PCR. e The level of miR-135a-5p expression in pulmonary artery smooth muscle cells (PASMCs) exposed to 3% O₂ (hypoxia) at the indicated time points. The levels of miR-135a-5p were normalized to that of 5S RNA. Data are presented as the means ± s.e.m. in a, b and c, d and the means ± s.d. in e. n = 4–6 rats for a, b and c, d; n = 3 independent experiments for e. ^*P < 0.05, ^**P < 0.01 vs. control in a, b, vs. 0 w in c, d, and vs. 0 h in e