Fig. 4.

TRPC1 is a direct target of miR-135a-5p in PASMCs. a Schematic representation of the TRPC1-3′-UTR with putative sites targeted by miR-135a-5p via Targetscan database. b PASMCs were treated as described in Fig. 2, and the protein expression of TRPC1 and β-actin was determined by Western blotting. c The combination between miR-135a-5p and the 3′-UTR sequence of rat TRPC1 mRNA was verified in PASMCs by luciferase reporter assay. d The effect of TRPC1 promoting the proliferation of PASMCs regulated by miR-135a-5p was determined by infection with TRPC1 including or lacking the 3′-UTR. Data are presented as the mean ± s.d. Normalized by the internal control, β-actin. n = 3 independent experiments. ^**P < 0.01 vs. normoxia in b, vs. wild type in c; ^*P < 0.05 vs. 3’-UTR-TRPC1 in d; ^##P < 0.01 vs. hypoxia