Fig. 5.

Suppression of polyclonal imatinib-resistant populations by sunitinib and regorafenib rapid alternation. PRISM analysis of barcoded cell lines was used to assess mixed GIST cultures treated with single-agent sunitinib, single-agent regorafenib, or rapid alternation (3 days of sunitinib alternating with 4 days of regorafenib). a Cell numbers were lowest in the rapid alternation arm, at all time-points. b Population profiling at day 28 demonstrated partial suppression of ATP-binding-pocket (V654A) and activation loop (D820A) imatinib-resistant GIST cells by the rapid alternation approach