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Methods for measuring tubulin kOn and kOff. (A) DIC-based measurements of microtubule growth rates and derivation of kOn and kOff rates (8). (B) Optical trap-based measurements of tip displacement and inference of kOn and kOff (13, 14). (C) iSCAT-based observation of single gold-labeled tubulin dimers and direct measurement of association and dissociation kinetics (2). DIC, differential interference contrast; MT, microtubule; TIRF, total internal reflection fluorescence; tub; tubulin.
