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. 2019 Mar 26;116(15):7288–7297. doi: 10.1073/pnas.1815027116

Fig. 2.

Fig. 2.

Activity-based di- and triUb probes. (A) Structures and synthesis of the diUb-NC1-PA2 probes and diUb-NC1-AMC2 fluorogenic substrates. (B) Generation of two types of hybrid triUb probes using a chemoenzymatic approach. TriUb-NC1-CL2 contains a noncleavable bond between the distal (light purple) and middle (blue) Ubs and a native isopeptide linkage between the middle and proximal (red) Ubs. TriUb-MA1-CL2 contains an MA warhead between the distal and middle Ubs and a native isopeptide linkage between the middle and proximal Ubs.