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. 2019 Mar 26;116(15):7288–7297. doi: 10.1073/pnas.1815027116

Fig. 3.

Fig. 3.

Interrogation of the role of β-hairpin in proximal Ub recognition (A) Superposition of the USP9X CD apo structure with CYLD in complex with M1-diUb (PDB ID code 3WXE). USP9X, CYLD, and diUb are colored orange, purple, and red, respectively. The CYLD and USP9X insertions are circled and labeled. (B) Cleavage activity of WT USP9X CD and deletion mutant Δ(1924–1943) assayed using IQF-diUb substrates of K11 and K63 linkages. (C) Labeling of WT and β-hairpin deletion mutant USP9X CD by K11 diUb-MA1 probe at different time points.