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. 2019 Apr 9;12:2649–2660. doi: 10.2147/OTT.S188054

Figure 2.

Figure 2

The effects of KCNQ1OT1 on L-OHP-induced protective autophagy and Atg4B expression levels in colon cancer cells.

Notes: (A) HCT116 and SW480 cells were treated with L-OHP with the absence and presence of autophagy inhibitor 3-MA or CQ, and then the expression levels of PARP and LC3 were detected by Western blot, with GAPDH as a loading control. *P<0.05, **P<0.01, and ***P<0.0001. (B) HCT116 and SW480 cells were transfected with siKCNQ1OT1 and treated with 10 μmol/L L-OHP for 24 h, and then Western blot was performed. *P<0.05, **P<0.01, and ***P<0.0001. (C) HCT116 and SW480 cells were treated with GFP-LC3 plasmid and then 10 μmol/L L-OHP was added. After treatment, cells were observed in the fluorescence microscope. (D) HCT116 and SW480 cells were co-transfected with siNC/siKCNQ1OT1 and pcDNA/pcDNA-Atg4B plasmids, and then cell viability assay was performed as above. *P<0.01 and **P<0.01. (E) HCT116 and SW480 cells were treated as shown in (D), and then the apoptosis rates were measured by flow cytometry. *P<0.05 and **P<0.01.

Abbreviations: CQ, chloroquine; L-OHP, oxaliplatin; 3-MA, 3-methyladenine; ns, not significant.