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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Glia. 2019 Feb 23;67(6):1076–1093. doi: 10.1002/glia.23589

Figure 6. Astrocyte activation via Gq and Gi/o DREADDs induces slow inward currents in neurons.

Figure 6.

(A) Representative excitatory postsynaptic current (EPSC) and slow inward current (SIC) recorded from a CA1 pyramidal neuron. (B) Representative traces showing SICs (asterisks) in control and in D-AP5. (C) Scheme of neuronal-astroglial synaptic elements and gliotransmission. (D) Scheme and representative traces showing SICs (asterisks) before and after ACh application. (E) Left: Mean number of SICs vs. time, binned at 10s (ACh was applied at t=0). T-test compares one minute before and one minute after agonist. Right: SIC frequency (per min) one minute before and one minute after ACh in different conditions. Statistical significance was determined by the Student’s t-test comparing mean values one minute before and after agonist application. (F-G) as in (D-E) but with CNO application to GqDREADD-expressing astrocytes. (H-I) as in (D-E) but with GABA application instead of ACh. (J-K) as in (D-E) but with CNO application to Gi/oDREADD-expressing astrocytes. Data are represented as mean ± SEM. P < 0.05 (*), P < 0.01 (**), and P < 0.001 (***).