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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Glia. 2019 Feb 23;67(6):1076–1093. doi: 10.1002/glia.23589

Figure 7. Astrocytic Gq and Gi/o DREADD activation increases neuronal action potential firing.

Figure 7.

(A and B) Representative trace of spontaneous action potential firing of CA1 pyramidal neuron before and after CNO application to GqDREADD-expressing astrocytes (A) and Gi/oDREADD-expressing astrocytes (B), and the normalized (from basal) firing frequency vs. time. CNO application was at t=0 (as in the other panels). (C and D) Representative trace (top) of action potential firing induced by short depolarizing pulses before and after CNO application to GqDREADD-expressing astrocytes (C) and Gi/oDREADD-expressing astrocytes (D), expanded traces (bottom left panels), and normalized firing frequency vs. time (bottom right panels). (E and F) Response to CNO in the presence of D-AP5. (G and H) Normalized firing frequency before and after CNO application to GqDREADD-expressing astrocytes (G) and GqDREADD-expressing astrocytes (H) in control and D-AP5. Data are represented as mean ± SEM. P < 0.05 (*).