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. Author manuscript; available in PMC: 2019 Jul 21.
Published in final edited form as: Mucosal Immunol. 2019 Jan 21;12(3):679–690. doi: 10.1038/s41385-019-0134-8

Figure 1. Induction of HMGB1 surface expression by platelets in response to exogenous and endogenous LTC4.

Figure 1.

Platelet rich plasma (PRP) was obtained from the indicated mouse strains and stimulated with various agonists. The CD41+ platelet gate was analyzed by flow cytometry with Abs specific for HMGB1 (left panels) or CD62P (right panels) along with corresponding isotype controls. A. Time dependent inductions of surface HMGB1 and CD62P by LTC4, LTD4, and LTE4 at the indicated concentrations. B. Comparison of LTC4 versus thrombin stimulation for surface expressions of HMGB1 and CD62P. C. Effect of Cysltr2 deletion on LTC4-induced HMGB1 and CD62P expression by platelets. D. Induction of HMGB1 and CD62P expression by platelet-derived LTC4 converted from exogenous LTA4. Results in A-D are mean ± SEM from three independent experiments that included a minimum of 3 mice per group.