C57BL/6, Irf3−/− and Irf3S1/S1 mice were exposed to the Gao-binge model as in Figure 1. A) Expression of CYP2E1 was measured by Western blot and normalized to HSC70. B) Formalin-fixed paraffin-embedded sections of liver were de-paraffinized and accumulation of 4-hydroxynonenal (4-HNE) adducts assessed by immunohistochemistry. Images were acquired at 10X. C) Phosphorylated-eIF2α, total eIF2α and CHOP10 protein in liver lysates was assessed by Western blot and normalized to HSC70. D) Expression of the spliced form of XBP1, as well as additional mRNA for ER stress markers, was measured in liver and normalized to 18S rRNA. Values represent means ± SEM, n=4–8 per group. Values with different superscripts are significantly different from each other, p<0.05, assessed by ANOVA..