Figure 5. TLR7/MyD88 signaling mediates miR-133a-3p mimic-induced leukocyte migration in vivo.

Wild-type (WT), TLR7^-/-, MyD88^-/-, or Trif^-/- male mice were administered i.p. with 20 μg of miR-133a-3p mimic or lipofectamine alone (Lipo.). Twenty hours later, the peritoneal lavage was harvested and the peritoneal cells were spun down. The total peritoneal cells were counted and the neutrophils (NE), resident macrophages (Mϕ), and recruited monocytes (MO) in the peritoneal cavity were determined by flow cytometry as detailed in Experimental Procedures. A, The total peritoneal cells in each group. B, The percentage of NE (Ly-6G⁺), Mϕ (F4/80^high), and MO (F4/80^low) among total peritoneal cells. Each error bar represents mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. n = 6 mice in MyD88^-/--Lipo Group, n = 4 mice in other Lipo Group, n = 6 mice in all miRNA group. C, Representative flow cytometry pictures gated for Ly-6G⁺, F4/80^high, and F4/80^low in each group. Lipo: lipofectamine; miR: miR-133a-3p.